Figure 9.

Cell ELISA to study the binding of the isozyme specific scFvs to the cell surface expressed PLAP. The two PLAP-specific purified scFvs, clone E4 and clone GL2D showed significant binding to the HeLa cells that express PLAP (solid bars). SaOs-2 cells, that do not express PLAP, served as control (empty bars). The scFv VB1 did not show binding to either of the cell types and served as a negative scFv control. The black bars on top indicate SD.