Table 5

Yield and STR genotypes from NTC samples

gDNA Input (ng)

Yield

N

Mean RT-PCR (ng)

Median ssDNA (%)

Median total DNA (ng)


gDNA

-

-

-

-

1

4

135

51.5

12230

10

4

0

50.8

11771

25

4

0

55

12340

50

4

24

45

12395

100

4

220

48.6

12686

200

4

0

54

14879


STR


gDNA Input (ng)

N

False Positive Rate (%)1

Mean Height Allele 1

Mean Height Allele 2


gDNA

64

6

93

93

1

128

5

2185

2199

10

128

0

-

-

25

128

0

-

-

50

128

92

375

393

100

128

92

571

571

200

128

2

2360

2360


1The number of "attempted" AmpFlSTR® Identifiler™ panel genotypes using gDNA NTCs and wgaDNA from NTC samples is 832 [two NTC DNA samples genotyped for gDNA in duplicate (64 possible genotype bins), and four wgaDNA samples from NTCs for each gDNA input level genotyped in duplicate (768 possible genotype bins)]. 2There were significantly more false positive STR genotypes in the wgaDNA produced from 50 ng and 100 ng gDNA inputs compared to the wgaDNA produced from 200 ng gDNA input (p = 0.01 and 0.02).

Bergen et al. BMC Biotechnology 2005 5:24   doi:10.1186/1472-6750-5-24

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