Table 3

Genotype Quality (GQ) scores of concordant STR genotypes

gDNA Input (ng)

GQ1Category, Heterozygotes

I

II

III

Total


gDNA

5

53

580

6382

1

43

137

256

4363

10

27

140

451

618

25

26

126

463

615

50

21

130

474

625

100

25

110

482

617

200

78

31

537

646


Total

225

727

3243

4195


gDNA Input (ng)

GQ Category, Homozygotes

I

II

III

Total


gDNA

0

9

204

2134

1

9

25

168

2025

10

2

8

202

212

25

1

8

199

208

50

4

11

196

211

100

8

10

191

209

200

8

20

184

212


Total

32

91

1344

1467


1Category I = GQ scores, originally ≥ 0.25 and <0.40, that have been successfully edited; Category II = GQ scores ≥ 0.40 and ≤ 0.50; Category III = GQ scores ≥ 0.79 and ≤ 0.90. 2GQ score distribution, heterozygote genotypes, gDNA versus all wgaDNA, p < 0.0001. 3GQ score distribution, heterozygote genotypes, wgaDNA produced from 1 ng gDNA input versus other gDNA inputs, p < 0.0001.4GQ score distribution, homozygote genotypes, gDNA versus wgaDNA produced from 1, 100, 200 ng gDNA input, p < 0.0001, 0.01, 0.0001, respectively. 5GQ score distribution, homozygote genotypes, wgaDNA produced from 1 ng gDNA input versus 10, 25, 50, 100 ng gDNA inputs, p = 0.0003, 0.0001, 0.009, 0.02, respectively.

Bergen et al. BMC Biotechnology 2005 5:24   doi:10.1186/1472-6750-5-24

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