Methodology article

A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renilla luciferase-antigen fusion proteins

Peter D Burbelo^*, Radoslav Goldman and Thomas L Mattson

Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University Medical Center, Washington, D.C. 20057

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BMC Biotechnology 2005, 5:22 doi:10.1186/1472-6750-5-22

Published: 18 August 2005

Additional File 3:

Table 3. Amount of protein (IgG) bound to A/G bead (μg/1 μl) from different sera used in this study

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Additional File 1:

Table 1. Mean and standard deviations of sera reactivity

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Additional File 2:

Table 2. Competition of antibody responses by unmodified antigens

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