Table 2

Correlation between hsp70i copy numbers in single blastomeres and whole 8-cell embryos, ± heat shock

hsp70i DNA copies
No-heat shock, No RT
Whole embryos (4)a
Per blastomere average ± s.d.
5 ± 2

hsp70i DNA + RNA copies
No-heat shock, + RT
Whole embryos (4)
Per blastomere average ± s.d.
19 ± 7
Heat shock, + RT


Embryo 1b
 Embryo 2c
Blastomere 1
 459
 750
Blastomere 2
 311
 705
Blastomere 3
 438
 281
Blastomere 4
 667
 730
Blastomere 5
 563
 491
Blastomere 6
1251
Per blastomere average ± s.d.
 488 ± 134
 701 ± 325



Whole embryosb (6)
Per blastomere average ± s.d
500 ± 172



Whole embryosc (2)
Per blastomere average

650

a The number of single embryos analyzed in each case is shown in parentheses.

b Heat shock followed by 2-hour recovery prior to analysis.

c Heat shock followed by 3-hour recovery prior to analysis.

Hartshorn et al. BMC Biotechnology 2005 5:2   doi:10.1186/1472-6750-5-2