Table 2

Correlation between hsp70i copy numbers in single blastomeres and whole 8-cell embryos, ± heat shock

hsp70i DNA copies


No-heat shock, No RT

Whole embryos (4)a

Per blastomere average ± s.d.

5 ± 2


hsp70i DNA + RNA copies


No-heat shock, + RT

Whole embryos (4)

Per blastomere average ± s.d.

19 ± 7


Heat shock, + RT

Embryo 1b

Embryo 2c

Blastomere 1

459

750

Blastomere 2

311

705

Blastomere 3

438

281

Blastomere 4

667

730

Blastomere 5

563

491

Blastomere 6

1251

Per blastomere average ± s.d.

488 ± 134

701 ± 325

Whole embryosb (6)

Per blastomere average ± s.d

500 ± 172

Whole embryosc (2)

Per blastomere average

650


a The number of single embryos analyzed in each case is shown in parentheses.

b Heat shock followed by 2-hour recovery prior to analysis.

c Heat shock followed by 3-hour recovery prior to analysis.

Hartshorn et al. BMC Biotechnology 2005 5:2   doi:10.1186/1472-6750-5-2

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