Table 2 |
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Correlation between hsp70i copy numbers in single blastomeres and whole 8-cell embryos, ± heat shock |
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hsp70i DNA copies |
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|
|
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|
No-heat shock, No RT Whole embryos (4)a Per blastomere average ± s.d. |
5 ± 2 |
|
|
|
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|
hsp70i DNA + RNA copies |
||
|
|
||
|
No-heat shock, + RT Whole embryos (4) Per blastomere average ± s.d. |
19 ± 7 |
|
|
|
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|
Heat shock, + RT |
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|
Embryo 1b |
Embryo 2c |
|
|
Blastomere 1 |
459 |
750 |
|
Blastomere 2 |
311 |
705 |
|
Blastomere 3 |
438 |
281 |
|
Blastomere 4 |
667 |
730 |
|
Blastomere 5 |
563 |
491 |
|
Blastomere 6 |
1251 |
|
|
Per blastomere average ± s.d. |
488 ± 134 |
701 ± 325 |
|
Whole embryosb (6) Per blastomere average ± s.d |
500 ± 172 |
|
|
Whole embryosc (2) Per blastomere average |
650 |
|
|
|
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a The number of single embryos analyzed in each case is shown in parentheses. b Heat shock followed by 2-hour recovery prior to analysis. c Heat shock followed by 3-hour recovery prior to analysis. |
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Hartshorn et al. BMC Biotechnology 2005 5:2 doi:10.1186/1472-6750-5-2 |
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