Table 1 |
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|
Comparison of four imaging methods to readout the wound healing assay |
|||||
|
Device |
Resolution |
Observable phenotypes |
Timepoint for assaying |
Acquisition speed |
Cost of device |
|
|
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|
Automated microscope |
3 μm/pixel |
Decreased wound healing, aberrant morphology, increased mitotic index |
7 and 24 hr |
~1 hr/plate with 4× ~1.5 hr/plate with 10× |
~$200,000 |
|
Fluorescence scanner |
42 μm/pixel |
Decreased wound healing |
7 and 24 hr |
26 minutes/plate |
~$50,000 |
|
Conventional scanner |
10 μm/pixel |
Decreased wound healing |
7 and 24 hr |
8.5 minutes/plate |
~$1,000 |
|
Fluorescence macroscope |
~100 μm/pixel |
Decreased wound healing |
24 hr |
5 seconds/plate |
~$500,000 |
|
|
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|
Yarrow et al. BMC Biotechnology 2004 4:21 doi:10.1186/1472-6750-4-21 |
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