GATA-stimulated pGL3-CYP11A reporter gene activities normalized by 2 different Renilla control plasmids. COS-7 cells were transfected with pGL3-CYP11A, and combinations of GATA-expressing and Renilla luciferase-encoding plasmids. Both firefly and Renilla luciferase activities were measured using the Dual Luciferase Reporter assay system. pGL3-CYP11A firefly luciferase activity is normalized by Renilla luciferase activity and expressed as fold change (mean ± sem) compared with control cells transfected with pcDNA3 and the corresponding Renilla plasmid.
Ho and Strauss BMC Biotechnology 2004 4:10 doi:10.1186/1472-6750-4-10