Promoter regions of pRL-TK and pRL-SV40. The Renilla luciferase gene (Rluc) is driven by the herpes simplex virus thymidine kinase (HSV-TK) promoter or SV40 enhancer/early promoter. Consensus GATA binding sequences, (A/T)GATA(A/G), are present in the intron upstream of T7 promoter and within HSV-TK but not SV40 promoter. Deletion mutants of the two control plasmids (pRL-TK-d238 and pRL-SV40-d238) were constructed by removal of 238 base pairs between the HindIII and ScaI restriction sites in the intron.
Ho and Strauss BMC Biotechnology 2004 4:10 doi:10.1186/1472-6750-4-10