Table 1

Chimeric primers for RT and primers for PCR.

Primers

Sequences


For RT

Rat VHL

Short

5'-CGGCTCGTGACTGCTATCTTCAATGTGATGTTGGCAAAAAT-3'

Long

5'-CGGCTCGTGACTGCTATCTTC(N)nAATGTGATGTTGGCAAAAAT-3'

Rat Collagen I

Short

5'-CGGCTCGTGACTGCTATCTTCCCTTGGCACCATCGTTGC-3'

Long

5'-CGGCTCGTGACTGCTATCTTC(N)nCCTTGGCACCATCGTTGC-3'

For PCR

Rat VHL

Upper

5'-GGGACCCATGATGGACTTCT-3'

Lower

5'-CGGCTCGTGACTGCTATCTTC-3'

Rat Collagen I

Upper

5'-CCCTGGTGAACGTGGTGTAC-3'

Lower

The same as the VHL lower primer


Gene-specific sequences: letters in italic; a universal sequence: bold letters; the linkers (N)n: either (A)10, (C)10, (G)10, (AT)5, or (A)5(G)5(A)5.

Shiao BMC Biotechnology 2003 3:22   doi:10.1186/1472-6750-3-22

Open Data