Application of the new quantitative RT-PCR to determine the ratios of gene expression among cell lines. The Collagen I cDNAs were synthesized by Omniscript reverse transcriptase. The observed 35-cycle values of long (L) over short (S) PCR products from different sample combinations were used for calculation of corrected ratios. 4N: wild-type VHL-transfected NRK; 6N: mutant VHL-transfected NRK.
Shiao BMC Biotechnology 2003 3:22 doi:10.1186/1472-6750-3-22