Observed and baseline-corrected ratios of long (L) to short (S) Collagen I and VHL PCR products. The cDNAs were synthesized by Omniscript reverse transcriptase from incremental quantities of total RNA (A). The observed ratios were corrected by values from either 1:1 or 5:1 RNA ratio of the corresponding PCR cycle. PCR efficiency plot is illustrated for Collagen I only (B).
Shiao BMC Biotechnology 2003 3:22 doi:10.1186/1472-6750-3-22