Mathematical model of PCR efficiency plot and estimation of original copy number after amplification. Accurate estimation can be obtained only when the PCR efficiency is known or is equal between two reactions. Top panel: two PCR reactions with the same original copy number but different amplification efficiency (A versus B). Bottom panel: two PCR reactions with different original copy number but the same amplification efficiency C.
Shiao BMC Biotechnology 2003 3:22 doi:10.1186/1472-6750-3-22