Table 1

Real-time nucleotide primer sequences





PRIMER CONCENTRATION &
 PCR EFFICIENCY *





GENE
 FORWARD PRIMER
 REVERSE PRIMER
 ACCESSION NUMBER
 PRODUCT LENGTH
 REFERNCE GENE
 TARGET GENE
 REFERNCE GENE
 TARGET GENE
hGAPDH
 AAC AGC GAC ACC CAT CCT C;
 CAT ACC AGG AAA TGA GCT TGA CAA
 NM_002046
 81



CODING
 CAC CTC TGG GCT ACG TGC TAG
 GAA CAC ATG CTG AGG TTT AAA GAG AAT
 NG_001332
 98
 50/50
 300/300
 -2.87
 -2.84
SIGNAL
 CAG AGG GGT GCC TCT GTC A
 GGA CCC CTC ACA AAG TGT CG
 NG_001332
 142
 100/100
 100/100
 -2.87
 -2.93
C-MYC
 TTC TAA GAG AAA TGT CCT GAG CAA TC
 TCA AGA CTC AGC CAA GGT TGT G
 X00364
 88
 100/100
 500/500
 -3.18
 -3.22
GLI
 CCC AAT CAC AAG TCA GGT TCC T
 CCT ATG TGA AGC CCT ATT TGC C
 NM_005269
 93
 100/100
 300/300
 -3.18
 -3.19
N-MYC
 CTT TCA AAT CGG TCC CCT GTC
 AGA GGT TCC AGG GTC GTC TT
 Y00664
 82
 50/50
 300/300
 -3.29
 -3.30
OPA1
 TGT GAT TGA AAA CAT CTA CCT TCC A
 TTT AAG CTT GAT ATC CAC TGT GGT GT
 NM_015560
 82
 50/50
 500/500
 -3.34
 -3.38
MGAPDH
 GTC TCC TGC GAC TTC AGC
 TCA TTG TCA TAC CAG GAA ATG AGC
 NM_008084
 107



HOP
 TGG AGT ACA ACT TCA ACA AGG TCA A
 GGA CTC ACC TGC GTC TGC TC.
 AF492703
 103
 50/50
 300/300
 -3.2
 -3.18
FLAG/HOP
 ATG GAC TAC AAG GAC GAT GAC CGA
 TGT TGA CCT TGT TGA AGT TGT ACT CC
 AF492703
 107
 50/50
 100/100
 -3.2
 -3.21

& Concentrations used in individual assay for F/R primers (nM). * PCR efficiency defined as the slope of the Log-linear phase of a dilution set as described in Figure 3A

Ponchel et al. BMC Biotechnology 2003 3:18   doi:10.1186/1472-6750-3-18