Table 1

Real-time nucleotide primer sequences

PRIMER CONCENTRATION &

PCR EFFICIENCY *


GENE

FORWARD PRIMER

REVERSE PRIMER

ACCESSION NUMBER

PRODUCT LENGTH

REFERNCE GENE

TARGET GENE

REFERNCE GENE

TARGET GENE


hGAPDH

AAC AGC GAC ACC CAT CCT C;

CAT ACC AGG AAA TGA GCT TGA CAA

NM_002046

81

CODING

CAC CTC TGG GCT ACG TGC TAG

GAA CAC ATG CTG AGG TTT AAA GAG AAT

NG_001332

98

50/50

300/300

-2.87

-2.84

SIGNAL

CAG AGG GGT GCC TCT GTC A

GGA CCC CTC ACA AAG TGT CG

NG_001332

142

100/100

100/100

-2.87

-2.93

C-MYC

TTC TAA GAG AAA TGT CCT GAG CAA TC

TCA AGA CTC AGC CAA GGT TGT G

X00364

88

100/100

500/500

-3.18

-3.22

GLI

CCC AAT CAC AAG TCA GGT TCC T

CCT ATG TGA AGC CCT ATT TGC C

NM_005269

93

100/100

300/300

-3.18

-3.19

N-MYC

CTT TCA AAT CGG TCC CCT GTC

AGA GGT TCC AGG GTC GTC TT

Y00664

82

50/50

300/300

-3.29

-3.30

OPA1

TGT GAT TGA AAA CAT CTA CCT TCC A

TTT AAG CTT GAT ATC CAC TGT GGT GT

NM_015560

82

50/50

500/500

-3.34

-3.38

MGAPDH

GTC TCC TGC GAC TTC AGC

TCA TTG TCA TAC CAG GAA ATG AGC

NM_008084

107

HOP

TGG AGT ACA ACT TCA ACA AGG TCA A

GGA CTC ACC TGC GTC TGC TC.

AF492703

103

50/50

300/300

-3.2

-3.18

FLAG/HOP

ATG GAC TAC AAG GAC GAT GAC CGA

TGT TGA CCT TGT TGA AGT TGT ACT CC

AF492703

107

50/50

100/100

-3.2

-3.21


& Concentrations used in individual assay for F/R primers (nM). * PCR efficiency defined as the slope of the Log-linear phase of a dilution set as described in Figure 3A

Ponchel et al. BMC Biotechnology 2003 3:18   doi:10.1186/1472-6750-3-18

Open Data