Loss of heterozygosity analysis of K-ras2 and p53 in Panc-1. There are approximately twice as many (A) K-ras2 polonies than (B) p53 polonies following the polony amplification of equal amounts of genomic DNA, likely indicating that one of two p53 alleles was lost. These results, coupled with the sequencing of mutational hotspots in these same genes, demonstrate that p53 experienced LOH.
Butz et al. BMC Biotechnology 2003 3:11 doi:10.1186/1472-6750-3-11