RT-PCR analysis of tomato transgenic floral buds. Analysis was performed with single strand cDNA synthesised from mRNA extracted from young flower buds of UC82 plants transformed with either DefH9-iaaM (panel b, lanes 1,2,3,4) or DefH9-RI-iaaM (panel a, lanes C3, C5, C6, C9, C10, C11; panel b, S3, S4, S5 and S6) gene. Either 0.05 fg (C3, C5, C6, C9, C10, C11 DefH9-RI-iaaM transgenic lines) or 0.2 fg (S3, S4, S5, S6 DefH9-RI-iaaM and 1, 2, 3, 4 DefH9-iaaM transgenic lines) of a 600 bp DefH9 cDNA fragment were used as internal standard in the PCR, giving an amplicon of 351 bp. The chimeric fragments are amplicons of 161 and 195 bp respectively, corresponding to the 5' end of the DefH9-iaaM and DefH9-RI-iaaM mRNAs.
Pandolfini et al. BMC Biotechnology 2002 2:1 doi:10.1186/1472-6750-2-1