Biophysiochemical properties of endothelial cells cultured on bio-inspired collagen films
1 Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk 790-784, Korea
2 Center for Biofluid and Biomimic Research, Department of Mechanical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-Gu, Pohang, Gyeongbuk 790-784, South Korea
3 Department of Biological Sciences, Center for Cognition and Sociality, Institute for Basic Science, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, South Korea
BMC Biotechnology 2014, 14:61 doi:10.1186/1472-6750-14-61Published: 2 July 2014
In this study, we investigated the effect of the extracellular matrix on endothelial dysfunction by careful observation of human umbilical vein endothelial cells (HUVECs) cultured on denatured collagen film.
HUVECs on denatured collagen film showed relatively high surface roughness compared with normal HUVECs. The expression levels of MMP-1, MMP-2 and CD146 increased in the ECs on denatured collagen film. In addition, we examined the accumulation of fluorescent beads on HUVEC layers subjected to circulatory flow. The number of accumulated fluorescent beads increased on the disorganized HUVEC layers.
The proposed in vitro study using bio-inspired collagen films could potentially be used in the size- and ligand-based design of drugs to treat endothelial dysfunction caused by circulatory vascular diseases.