Open Access Highly Accessed Research article

Simultaneous multi-analyte urinary protein assay for bladder cancer detection

Charles J Rosser123*, Yunfeng Dai4, Makito Miyake1, Ge Zhang1 and Steve Goodison125

Author Affiliations

1 MD Anderson Cancer Center Orlando, Orlando, FL, USA

2 Nonagen BioScience Corp, Jacksonville, FL, USA

3 University of Hawaii Cancer Center, Honolulu, HI, USA

4 Department of Biostatistics, The University of Florida, Gainesville, FL, USA

5 Department of Health Sciences Research, Mayo Clinic, Jacksonville, FL, USA

For all author emails, please log on.

BMC Biotechnology 2014, 14:24  doi:10.1186/1472-6750-14-24

Published: 1 April 2014



The ability to accurately measure multiple proteins simultaneously in a single assay has the potential to markedly improve the efficiency of a myriad of clinical assays. Here, we tested the performance of a new, multiplex protein array platform to quantitate three bladder cancer-associated proteins in urine samples. The following analytes, interleukin 8 (IL8), matrix metallopeptidase 9 (MMP9), and vascular endothelial growth factor A (VEGFA) were monitored using Q-plex, a customized multiplex ELISA system from Quansys Biosciences, and individual target commercial ELISA kits. The performance of the two approaches was compared by evaluating the diagnostic accuracy of the biomarker assays in samples from a cohort of 73 subjects of known bladder cancer status.


The combination biomarker panel analyses revealed an AUROC value of 0.9476 for the Q-plex assay, and 0.9119 for the combination of the single-target ELISA assays. The Q-plex assay achieved an overall diagnostic sensitivity of 0.93 and specificity of 0.81, and the individual target ELISA assays achieved an overall sensitivity of 0.77 and specificity of 0.91.


Based on these encouraging preliminary data, we believe that the Q-Plex technology is a viable platform that can be exploited as an efficient, highly accurate tool to quantitate multiplex panels of diagnostic proteins in biologic specimens.

Biomarkers; Bladder cancer; Q-plex; Protein; Urine