Table 2

The 8 bp window motif analyses for several DNA polymerase experiments
Top 1% (655 in 65,536) 8 bp window motif analyses for several DNA polymerase experiments
QTT-A QTT-AR PGT-A PGT-AR QTT-A63 QTT-A65 QTT-A67 QHH-B
(GC,CG) motif at 3’ end of primer 88% 89% 90% 90% 90% 91% 88% 25%
(GC,CG) motif at 5’ end of 6 N portion of the primer 8% 6% 8% 11% 12% 10% 8% 89%
(TC) motif at first 2 bp of runway 3% 4% 3% 3% 4% 4% 4% 19%
(CC) motif at first 2 bp of runway 28% 26% 28% 30% 23% 23% 25% 30%
(CGC,CCG,GCG, GGC,TGC,TCG) motif at 3’ end of primer 87% 88% 88% 89% 90% 90% 87% 22%
(AGC,ACG) motif at 3’ end of primer 1% 1% 1% 1% 1% 1% 1% 4%
(ACG) motif at 3’ end of primer 1% 1% 1% 1% 1% 1% 1% 2%
(AGC) motif at 3’ end of primer 0 0 0 0 0 0 0 2%

The motif percentage breakdown for the top 1% of ranked sequences are shown for several PCR experiments: QTT-A touchdown, QTT-AR, PGT-A, PGT-AR, QTT-A63, QTT-A65, QTT-A67, and the QHH-B touchdown PCR amplification experiment.

Pan et al.

Pan et al. BMC Biotechnology 2014 14:10   doi:10.1186/1472-6750-14-10

Open Data