Figure 6.

Affect of annealing temperature on the amplified library. Amplification was performed at 63, 65, and 67°C, and the sequenced reads were compared to touch down PCR experiments (70-60°C) for the 6 bp observation window.

Pan et al. BMC Biotechnology 2014 14:10   doi:10.1186/1472-6750-14-10
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