Importance of the 3’ end of the primer sequence on amplification bias. The OBV for the 6 bp window of (n = 4,096) was plotted against the OBV of the sequence of the reverse trimer order. For instance the OBV for the 6-mer “CCG AGG” is plotted against the OBV of “AGG CCG”. Although the sequences have the same GC content, the amplification results are not correlated.
Pan et al. BMC Biotechnology 2014 14:10 doi:10.1186/1472-6750-14-10