Open Access Highly Accessed Retraction

Retraction: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10

Ondrej Kaplan1, Karel Bezouska12, Ondrej Plihal1, Rudiger Ettrich3, Natallia Kulik3, Ondrej Vanek12, Daniel Kavan12, Oldrich Benada1, Anna Malandra14, Ondrej Sveda1, Alicja B Vesela1, Anna Rinagelova1, Kristyna Slamova1, Maria Cantarella4, Jurgen Felsberg1, Jarmila Duskova5, Jan Dohnalek5, Michael Kotik1, Vladimir Kren1 and Ludmila Martinkova1*

Author Affiliations

1 Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20, Prague, Czech Republic

2 Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-128 40, Prague, Czech Republic

3 Centre of Biocatalysis and Biotransformation, Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, Zámek 136 373 33, Nové Hrady, Czech Republic

4 Department of Chemistry, Chemical Engineering and Materials, University of L'Aquila, Via Campo di Pile - Zona industriale di Pile, I-67100, L'Aquila, Italy

5 Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského náměstí 2, CZ-162 06, Prague, Czech Republic

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BMC Biotechnology 2013, 13:57  doi:10.1186/1472-6750-13-57

The electronic version of this article is the complete one and can be found online at:

Received:12 July 2013
Accepted:12 July 2013
Published:20 July 2013

© 2013 Kaplan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The authors have retracted this article [1] due to the ethical misconduct of Karel Bezouska and misappropriation of data which fundamentally changes the main findings of the article. Specifically, the upper amino acid sequence in Figure 1 (by KB) in BMC Biotechnol 11:2 was incorrect, therefore Nit-ANigWT and Nit-ANigRec (nitrilase expressed in E. coli harbouring a nit gene from A. niger K10) were not variants of the same enzyme as hypothesized in the original article. The different biochemical properties of Nit-ANigWT and Nit-ANigRec discussed in the paper were caused by a significant difference in the primary structures of these enzymes.


  1. Kaplan O, Bezouska K, Plihal O, Ettrich R, Kulik N, Vanek O, Kavan D, Benada O, Malandra A, Sveda O, Vesela AB, Rinagelova A, Slamova K, Cantarella M, Felsberg J, Duskova J, Dohnalek J, Kotik M, Kren V, Martinkova L: Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10.

    BMC Biotechnol 2011, 11:2. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL