Comparison of self-contained and autoregulated OneVector System. (A) Determination of luciferase activity of Ht1080 cell populations transduced by either MOV-scT6 or MOV-scT6cA and enriched by FACS. The left panel shows the luciferase activity determined in the on/off state of the system after transduction with low MOI, the right panel after transduction with high MOI. Two independent populations were generated at the indicated condition and each was measured twice. Induction was calculated from the luciferase activities determined in the on/off state and given above the bars. (B) Northern blot analysis of representative Ht1080 cell populations transduced by either MOV-scT6 or MOVscT6cA. The blot was probed for the M2 transactivator or GAPDH.
Heinz et al. BMC Biotechnology 2013 13:5 doi:10.1186/1472-6750-13-5