Shuttle vector pMC2 used for the expression of H. lacusprofundi bga in Halobacterium sp. NRC-1. Location and transcriptional orientation of bla, β-lactamase for ampicillin resistance; mev, HMG-CoA reductase for mevinolin resistance; bga, β-galactosidase, and rep, the Halobacterium pGRB replicase gene are shown. Position of the temperature induced promoter, Pcs→ and KpnI, NdeI and BamHI restriction sites are indicated.
Karan et al. BMC Biotechnology 2013 13:3 doi:10.1186/1472-6750-13-3