Comparison of copy numbers among transgenic T0 plants transformed with HPT-GFP vectors controlled by the 35S, Nos or tCUP1 promoters. Southern blot analysis of integrated T-DNA copy number in T0 transgenic plants transformed with 35S (A), Nos (B) and tCUP1 (C) promoter controlled HPT-GFP vectors using an HPT probe. DNA was extracted from leaves of 4 lines with the lowest GFP expression (1 to 4) and 4 lines with the highest GFP expression (17 to 20 in red colors) in each transformation according to the GFP expression analysis shown in Figure 5. About 3 μg of NdeI digested genomic DNA was loaded for each lane. Lines indicated with “*” were selected for further GFP observation in the T1 generation.
Zhou et al. BMC Biotechnology 2013 13:29 doi:10.1186/1472-6750-13-29