Effect of rhTF on the proliferation of HL-60 cells. The viable cell concentration of HL-60 cells after three days culture in serum-free medium supplemented with no hTF, 0.005, 0.05, 0.5, 5 and 50 mg/L of hTF (holo-form, from Sigma) or rhTF (Partial; partially iron saturated) was determined by fluorescence assay. Data is represented as average with error bars indicating standard deviation, n = 3.
Zhang et al. BMC Biotechnology 2012 12:92 doi:10.1186/1472-6750-12-92