Binding specificity of selected anti-V3 scFv clones. The binding of scFv antibodies was determined by ELISA against V3 peptides of clade C and B, MPER and ID peptides of HIV, peptide pool (unrelated viruses) and BSA. The clones 3E6B and 3E7B bound specifically to V3 peptides. The experiment was performed in triplicates.
Kumar et al. BMC Biotechnology 2012 12:87 doi:10.1186/1472-6750-12-87