Localization of scFv antibody in different fractions. Lane M, protein molecular weight marker ; lane L, cell lysate fraction; lane S, culture supernatant; lane P1 and P2 are, periplasmic fraction (20 μl and 10 μl respectively loaded); lane I, inclusion body (insoluble) fraction. Lane –Ve control, cell lysate of phagemid vector without scFv was used.
Kumar et al. BMC Biotechnology 2012 12:87 doi:10.1186/1472-6750-12-87