Phage ELISA binding specificity. Selection of clones exhibiting binding to the V3 peptides of clade C and clade B HIV-1. ELISA wells were coated with the V3C and V3B peptide. MPER peptide, ID loop peptide, BSA and a peptide pool of unrelated viruses were used as negative controls. The experiment was done in duplicates and repeated at least twice and the mean OD values are shown. Clones showing O.D three times the negative control was considered as positive.
Kumar et al. BMC Biotechnology 2012 12:87 doi:10.1186/1472-6750-12-87