DNA fingerprinting analysis of scFv clones. Ten scFv gene fragments were amplified by PCR and digested with BstN1 at 60°C for 3 hours. Restriction pattern was analysed on 2% agarose gel. Arrows indicate that clones 3 and 6 exhibit identical DNA fingerprinting pattern. Lane M, DNA ladder (100 bp).
Kumar et al. BMC Biotechnology 2012 12:87 doi:10.1186/1472-6750-12-87