Table 5

Nucleofection results PKC2109, PKCm, PFF26 and PEF0110
Cell Number DNA amount [μg] Program Quality Cells in suspension Fluor. intensity Transfection efficiency [%]
PKC2109
0.5x106 2 A24 2 1 + 28
2 U23 2 2 + 60
2 U12 1 1 ++ 63
2 T16 2 1 + 54
2 V13 1 1 ++ 59
0.5x106 1 U12 1 1 + 36
5 U12 1 1 ++ 70
10 U12 1 1 +++ 77
20 U12 2 1 ++ 89
1.0x106 5 U12 1 1 ++ 68
10 U12 1 1 +++ 88
20 U12 2 2 +++ 83
PKCm
0.5x106 2 U12 1 1 ++ 49
2 T16 1 1 ++ 50
2 V13 2 1 +++ 66
PFF26
0.5x106 2 U12 1 1 + 53
2 T16 1 1 + 50
2 V13 2 2 ++ 77
PEF0110
0.5x106 2 U12 1 1 + 15
2 T16 1 1 + 11
2 V13 2 2 + 34

Scale for the assessment of cell quality: 1- excellent-good; 2- cells are bit stretched/strongly fibroblast-like; 3- stretched cells with vacuoles; 4- cells with very spindle-shaped morphology 5- extreme spindle-shaped with vacuoles; Scale for the assessment of cells in suspension: 0- none; 1- low number of cells; 2- a lot of cells; Fluor. = Fluorescence.

Richter et al.

Richter et al. BMC Biotechnology 2012 12:84   doi:10.1186/1472-6750-12-84

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