Open Access Research article

Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig

Anne Richter1, Mayuko Kurome12, Barbara Kessler1, Valeri Zakhartchenko1, Nikolai Klymiuk1, Hiroshi Nagashima2, Eckhard Wolf12 and Annegret Wuensch1*

Author Affiliations

1 Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians-Universität München, Feodor-Lynen-Straße 25, Munich, 81377, Germany

2 Meiji University International Institute for Bio-Resource Research, Kawasaki, Japan

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BMC Biotechnology 2012, 12:84  doi:10.1186/1472-6750-12-84

Published: 9 November 2012

Additional files

Additional file 1:

Single cell clone colonies of PKC2109 at P3. Single cell colonies were generated and analyzed after 5–8 days. The cells and formed colonies differed morphologically: fibroblast-like cells [A, B, C, D, F], epithelial- and endothelial-like cells [E], cell size (smaller [A]), colony compactness (cells very close [A, C, E, F], gaps between cells [B, D]) and colony shape (clearly defined [A, C], frayed colonies [B, D, F]). Scale bar = 100 μm.

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