Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig
1 Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians-Universität München, Feodor-Lynen-Straße 25, Munich, 81377, Germany
2 Meiji University International Institute for Bio-Resource Research, Kawasaki, Japan
BMC Biotechnology 2012, 12:84 doi:10.1186/1472-6750-12-84Published: 9 November 2012
Additional file 1:
Single cell clone colonies of PKC2109 at P3. Single cell colonies were generated and analyzed after 5–8 days. The cells and formed colonies differed morphologically: fibroblast-like cells [A, B, C, D, F], epithelial- and endothelial-like cells [E], cell size (smaller [A]), colony compactness (cells very close [A, C, E, F], gaps between cells [B, D]) and colony shape (clearly defined [A, C], frayed colonies [B, D, F]). Scale bar = 100 μm.
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