Table 2

Role of medium supplements
LDH release (%)
Solution Without deferoxamine With deferoxamine
RPMI 66 ± 26 01 ± 1
RPMI without supplements 76 ± 23 02 ± 1

Vero-B4 cells were incubated at 4°C in RPMI 1640 supplemented with fetal bovine serum (10%) and penicillin/streptomycin (25 U ml-1/25 μg ml-1) or in “pure” RPMI 1640 without supplements for 168 h. The iron chelator deferoxamine (1 mM) was added to part of the incubations. Lactate dehydrogenase (LDH) release was assessed after cold storage (n=4).

Pless-Petig et al.

Pless-Petig et al. BMC Biotechnology 2012 12:73   doi:10.1186/1472-6750-12-73

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