Influence of modified Krebs-Henseleit buffer on cold-induced injury to Vero-B4 cells. Vero-B4 cells were stored at 4°C for 168 hours in RPMI 1640, Krebs-Henseleit buffer (KH), modified KH buffer with either 11.1 mM glucose (KHG), low Ca2+ concentration (KH(Ca-); 0.42 mM) or high inorganic phosphate concentration (KH(P+); 5.6 mM) alone or in different combinations (KHG(Ca-,P+), KHG(Ca-), KHG(P+), KH(Ca-,P+)). Some cells were stored in the presence of the iron chelator deferoxamine (1 mM, striped bars). Cell injury was assessed by the release of lactate dehydrogenase (LDH; n = 4) directly after cold storage (168 h).
Pless-Petig et al. BMC Biotechnology 2012 12:73 doi:10.1186/1472-6750-12-73