Cold-induced injury to Vero-B4 cells after cold incubation in different cell culture media. Vero-B4 cells were incubated in RPMI 1640, DMEM, L-15 and M199 at 4°C for 168 hours and then rewarmed in RPMI 1640 at 37°C for 3 hours. Cell injury was assessed by release of lactate dehydrogenase (LDH). Filled symbols represent media without, open symbols with 1 mM deferoxamine (n = 4; ** p < 0.01 vs. RPMI 1640 at 171 h).
Pless-Petig et al. BMC Biotechnology 2012 12:73 doi:10.1186/1472-6750-12-73