Cold-induced injury to Vero-B4 cells. Vero-B4 cells were incubated in RPMI 1640 (RPMI), Krebs-Henseleit buffer (KH) and KH + 11.1 mM D-glucose (KHG) at 4°C for 168 hours and then rewarmed in RPMI 1640 at 37°C for 3 hours. Part of the cells were incubated in the presence of the iron chelator deferoxamine (+ Def.; 1 mM; open symbols). Cell injury was assessed by release of lactate dehydrogenase (LDH; n = 4; ** p < 0.01 vs. RPMI 1640 at 171 h).
Pless-Petig et al. BMC Biotechnology 2012 12:73 doi:10.1186/1472-6750-12-73