Figure 4.

KO cell lines were confirmed by western blot. (a) Western blots of wild-type, heterozygous and homozygous SirT1 KO cell lines from HCT116 cells using anti-SirT1 and anti-β-actin antibodies. WT indicates parental cells, WT/KO indicates SirT1 heterozygous KO cells, KO/KO indicates SirT1 homozygous KO cells. (b) Western blots of wild-type and homozygous HDAC2 KO cell lines from DLD1 cells using anti-HDAC2 and anti-β-actin antibodies. WT indicates parental cells, WT/KO indicates HDAC2 heterozygous KO cells, KO/KO indicates HDAC2 homozygous KO cells.