Construction of a somatic cell KO vector via a one-step cloning method. Cassette A is indicated by two rectangles and cassette B by two echelons. The homologous arms were amplified using PCR with primers containing the 15–18 nt sequence at the 5′ end and sequences specific for the ExoIII-generated overhangs in pAAV-LIC. E2–6: exons 2–6; NEO: neomycin resistance gene; TK: thymidine kinase promoter.
Liu et al. BMC Biotechnology 2012 12:71 doi:10.1186/1472-6750-12-71