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Open Access Highly Accessed Methodology article

A one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines

Yi Liu, Shangze Li, Huihui Zhang, Zurong Wan, Xiaodong Zhang and Runlei Du*

BMC Biotechnology 2012, 12:71  doi:10.1186/1472-6750-12-71

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