Table 4

Cell disruption conditions predicted by RSM and validation of the experimental data for cell disruption capability and specific protein release of HBsAg by optimal cell disruption strategy
Optimal cell disruption strategy Glass bead cell disruption
Parameters
Biomass concentration (g/L) 7.76 7.70
Number of passes (times) 20 -
Pulse pressure (bar) 1,029 -
Vortexing (times) - 20
Glass beads (g/L) - 40
Results
CDR (%) 75.68 37.18
Specific protein (mg/L) 29.20 ± 0.96 7.28 ± 1.06

Tam et al.

Tam et al. BMC Biotechnology 2012 12:70   doi:10.1186/1472-6750-12-70

Open Data