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Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology

Yew Joon Tam, Zeenathul Nazariah Allaudin*, Mohd Azmi Mohd Lila, Abdul Rani Bahaman, Joo Shun Tan and Morvarid Akhavan Rezaei

BMC Biotechnology 2012, 12:70  doi:10.1186/1472-6750-12-70