Additional file 2.


Video 2: 293T cells transfected with 0.4 μg of GFP + 0.4 μg Env plasmid. Cells were kept in FCS-containing medium for the whole duration of the footage, which spans a 27-hour period from 19 h to 46 h post transfection. The video shows a marked fusion process (compare to Additional video 1), including visible cell-to-cell GFP transfer, while cells keep dividing over time. More details are provided in the article main text. Speed: x 4200.

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Bourret et al. BMC Biotechnology 2012 12:69   doi:10.1186/1472-6750-12-69