Open Access Methodology article

Development of an improved polykaryon-based influenza virus rescue system

Vincent Bourret123*, Jon Lyall4, Mariette F Ducatez23, Jean-Luc Guérin23 and Laurence Tiley4

Author Affiliations

1 Cambridge Infectious Disease Consortium, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK

2 INRA, UMR 1225, IHAP, Toulouse, F-31076, France

3 Université de Toulouse, INP, ENVT, Toulouse, F-31076, France

4 Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK

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BMC Biotechnology 2012, 12:69  doi:10.1186/1472-6750-12-69

Published: 25 September 2012

Additional files

Additional file 1:

Video 1: 293T cells transfected with 0.4 μg of GFP + 0.4 μg of inert B1 plasmid. Cells were kept in FCS-containing medium for the whole duration of the footage, which spans a 27-hour period from 19 h to 46 h post transfection. More details are provided in the article main text. Speed: x 4200.

Format: AVI Size: 1.7MB Download file

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Additional file 2:

Video 2: 293T cells transfected with 0.4 μg of GFP + 0.4 μg Env plasmid. Cells were kept in FCS-containing medium for the whole duration of the footage, which spans a 27-hour period from 19 h to 46 h post transfection. The video shows a marked fusion process (compare to Additional video 1), including visible cell-to-cell GFP transfer, while cells keep dividing over time. More details are provided in the article main text. Speed: x 4200.

Format: AVI Size: 1.6MB Download file

Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more

Open Data