Structural insights from random mutagenesis of Campylobacter jejuni oligosaccharyltransferase PglB
- Equal contributors
1 Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomaterials, CH-9014, St. Gallen, Switzerland
2 GlycoVaxyn AG, CH-8952, Schlieren, Switzerland
BMC Biotechnology 2012, 12:67 doi:10.1186/1472-6750-12-67Published: 24 September 2012
Additional file 1:
Validation of the 96-well DWP-ELISA screening system. Sandwich ELISA read-outs for EPA-CP5 glycoproteins and final optical densities (600 nm) of 90 wells inoculated with individual clones of E. coli CLM24 (pGVXN345-pGVXN150-pGVXN112Kan) (wt PglB, open symbols) and 6 distributed wells inoculated with E. coli CLM24 (pGVXN345-pGVXN150-pGVXN113Kan) (inactive PglB, closed symbols). ELISA absorbance at 450 nm was measured against air; OD600 was corrected for optical density of sterile medium.
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