Figure 4.

ELISA characterisation of rPlnD1.A: Immunoreactivity of protein core using monoclonal anti-perlecan domain I (A71) and polyclonal anti-perlecan antibody (CCN-1); B: HS GAG characterization using monoclonal antibodies against HS chains (10E4), HS/heparin (2Q546), heparin (A7.10) and HS stub detection (light bars) after digestion with Hep III (3G10); C: CS GAG chain characterization using monoclonal antibodies against CS tetrasaccharide type A-D (CS-56), CS hexasaccharide type C-C-A (LY111), CS hexasaccharide type C-A-D (MO-225), CS (7D4) and CS stubs (1B5 (unsulfated), 2B6 (4S) and 3B3 (6S)) detected after no treatment (dark bars), or chondroitinase ABC digestion (light bars). Data corrected for background absorbance and presented as mean ± standard deviation (n=3).

DeCarlo et al. BMC Biotechnology 2012 12:60   doi:10.1186/1472-6750-12-60
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