ELISA detection of rotavirus strain INDIANA (SbI, PG6) using larva-derived VHHs. Serial dilutions of purified or raw material from larvae expressing monomeric VHH 3B2 or 2KD1 were tested and compared with the VHH obtained from E. coli. The cut-off point of the assay was established at an OD405nm of 0.0664.
Gómez-Sebastián et al. BMC Biotechnology 2012 12:59 doi:10.1186/1472-6750-12-59