Table 1

Percent hydroxylation of proline in bovine collagen III and in recombinant human collagen III
LC-MS Assay (%) Amino Acid Analysis (%)
Bovine collagen III (as-received) 38 ± 1.1 37 ± 2.7
Bovine collagen III (extracted from SDS-PAGE) 36 ± 3.4 n/a
Recombinant human collagen III N.D.* N.D.*
  No hydroxylase (p4Ha, p4Hb) genes
  Collagen on 2-micron plasmid
Recombinant human collagen III 5.8 ± 0.5 5.2 ± 1.3
  CEN/ARS–p4Ha–p4Hb with SUC2 signal
  Collagen on 2-micron plasmid
Recombinant human collagen III 12 ± 1.8 12§
  CEN/ARS–p4Ha–p4Hb with SUC2 signal
  Collagen on CEN/ARS plasmid

* "N.D." denotes no significant signal of hydroxyproline above background noise.

§Only 1 AAA result available.

Chan et al.

Chan et al. BMC Biotechnology 2012 12:51   doi:10.1186/1472-6750-12-51

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