Additional file 6.
Figure S3. Comparison of mixed PNA and morpholino phenotypes. Embryos were injected with 400 μM Six3mix-PNA (PNA) or 100 μM Six3-MO (MO) and at stage 20 analysed by in situ hybridisation with probes for pax2 and rx2. Typical results are shown for each group of weak, moderate and strong phenotypes, wildtype (WT) embryos are shown as a reference. An arrowhead marks pax2 expression in the mid-hindbrain boundary, note the reduced expression in the strong group. The eye vesicles in the weak group are smaller compared to wildtype embryos, in the moderate group their size is further reduced, in the strong group no eye vesicles were detectable.
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Dorn et al. BMC Biotechnology 2012 12:50 doi:10.1186/1472-6750-12-50