Table 1

Freezing protocols used
Protocol Description Duration [min] References
1 0,3°C/min to −100°C 347
Store in vapour phase at −134°C [47]*
2 1°C/min to −80°C 84 [46-50]
Store in vapour phase at −134°C
3 1°C/min to - 30°C 44
5°C/min to −80°C [44,51]*
xStore in vapour phase at −134°C
4 1°C/min to −20°C 34
5°C/min to −40°C
10°C/min to −80°C Designed
20°C/min to −100°C
Store in vapour phase at −134°C
5 1°C/min to −6°C 26
25°C/min to −50°C Designed
10°C/min to −90°C
Store in vapour phase at −134°C
6 Directly into the vapour phase 0
7 99°C/min to −100°C 2
Store in vapour phase at −134°C [35,52]*

* Modified.

Naaldijk et al.

Naaldijk et al. BMC Biotechnology 2012 12:49   doi:10.1186/1472-6750-12-49

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