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Open Access Highly Accessed Methodology article

An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy

Yanzhen Bi*, Xianfeng Qiao, Zaidong Hua, Liping Zhang, Ximei Liu, Li Li, Wenjun Hua, Hongwei Xiao, Jingrong Zhou, Qingxin Wei and Xinmin Zheng*

BMC Biotechnology 2012, 12:39  doi:10.1186/1472-6750-12-39

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