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Open Access Highly Accessed Methodology article

pMAA-Red: a new pPZP-derived vector for fast visual screening of transgenic Arabidopsis plants at the seed stage

Muhammad Amjad Ali1, Kausar Hussain Shah1 and Holger Bohlmann12*

Author Affiliations

1 Division of Plant Protection, Department of Crop Sciences, University of Natural Resources and Life Sciences, Vienna, Austria

2 Division of Plant Protection, Department of Crop Sciences, University of Natural Resources and Life Sciences, UFT Tulln, Konrad Lorenz Strasse 24, 3430, Tulln, Austria

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BMC Biotechnology 2012, 12:37  doi:10.1186/1472-6750-12-37

Published: 2 July 2012

Abstract

Background

The production of transgenic plants, either for the overproduction of the protein of interest, for promoter: reporter lines, or for the downregulation of genes is an important prerequisite in modern plant research but is also very time-consuming.

Results

We have produced additions to the pPZP family of vectors. Vector pPZP500 (derived from pPZP200) is devoid of NotI sites and vector pPZP600 (derived from pPZP500) contains a bacterial kanamycin resistance gene. Vector pMAA-Red contains a Pdf2.1: DsRed marker and a CaMV:: GUS cassette within the T-DNA and is useful for the production of promoter: GUS lines and overexpression lines. The Pdf2.1 promoter is expressed in seeds and syncytia induced by the beet cyst nematode Heterodera schachti in Arabidopsis roots. Transgenic seeds show red fluorescence which can be used for selection and the fluorescence level is indicative of the expression level of the transgene. The advantage is that plants can be grown on soil and that expression of the marker can be directly screened at the seed stage which saves time and resources. Due to the expression of the Pdf2.1: DsRed marker in syncytia, the vector is especially useful for the expression of a gene of interest in syncytia.

Conclusions

The vector pMAA-Red allows for fast and easy production of transgenic Arabidopsis plants with a strong expression level of the gene of interest.

Keywords:
Transient expression; pPZP family vectors; Marker gene; Agroinfiltration; DsRed; Agrobacterium; Arabidopsis transformation